Name: Pinpin Long

Date of birth: August 17, 1994

Nationality: Chinese

Personal: Married

Telephone: 86-18062551062

Email: longpinpintjmu@163.com

Address: Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology (HUST), Wuhan 430030, China

My research to date has focused on the interaction of environmental exposures (metals, nutrients) and genetic factors (genetics, epigenetics) on the risk of incident cardiovascular disease. I have investigated the associations of metal exposures with cardiovascular disease and its related traits in populations with different genetic backgrounds based on the gene-environment interaction method. Furthermore, I have investigated how metal exposures affect the occurrence and development of cardiovascular disease by changing the level of DNA methylation and gene expression in the body. I also have interests in the role and underlying mechanisms of the one-carbon metabolic pathway in the development of cardiovascular diseases. I have also worked on the inference of causal associations through Mendelian randomization using in-house or public gene database.

· Produce high quality publishable research which furthers the knowledge and understanding of the influence of the interaction between environmental factors and genetic factors on the occurrence and development of cardiovascular disease

· Elucidate the role and potential mechanism of DNA methylation in the occurrence of cardiovascular disease caused by environmental exposure, providing promising intervention targets for the primary prevention of cardiovascular disease

· Active teaching commitments in the fields of occupational health, environmental health, analytical chemistry, and epidemiology

· Disseminating research findings via academic publications and presentations to both academic and public audiences

Doctoral Degree of Medicine, Occupational and Environmental Health, (2016.9-2021.6)

School of Public Health, Tongji Medical College, Huazhong University of Science and Technology

Supervisor: Professor Tangchun Wu

Bachelor of Natural Science, Health Inspection Techniques, (2012.9-2016.6)

West China School of Public Health, Sichuan University

Bachelor of Management,  Financial management, (2014.9-2016.6)

Postdoctoral Fellow/Research Assistant (2021.7-present), Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College,  Huazhong University of Science and Technology

Technology

Supervisor: Professor Tangchun Wu

Research skills

· Managing a research project from conception to writing up

· Experience of working in field follow-up of cohort study populations, including questionnaires, medical examinations, and biological sample collection (Dongfeng-Tongji cohort, Wuhan-Zhuhai cohort)

· Cleaning large epidemiological datasets and conducting research designs such as cohort studies, nested case-control studies, case-cohort studies, etc

· Statistical analysis (using Linux, Stata, SPSS, SAS and R software packages): generalized linear regression; logistic regression; cox proportional hazards regression; case-cohort proportional hazard model; lasso or elastic net regression; ROC analysis; genome-wide association study; epigenome-wide association study; eQTL, meQTL, Mendelian randomization analysis; gene-environment interaction study; mediation analysis, etc

· Experiment technique: measurement of pollutant (ICP-MS, HPLC-MS); gene chip detection (whole genome chip detection technology, whole genome methylation chip detection technology); qPCR; the extraction and purification of DNA and RNA; RNA sequencing library construction, etc.

· Conducting meta-analysis systematic literature reviews

· Extracting data from public databases (GEO, BBJ, UKB, etc) and performing data mining such as two-stage Mendelian randomization analysis, in accordance with standard protocol

· Disseminating research findings via academic publications and presentations to both academic and public audiences

· Providing methodological and statistical support for clinicians

Other skills

• IT      skills: bibliographic software (Mendeley,      Endnote); Microsoft Office (Word, Excel, Powerpoint); English ability (CET6)

• Journal      reviewer: Environment International, Environmental Pollution, Ecotoxicology and Environmental Safety,      Circulation: Cardiovascular Genetics, Public Health Nutrition, Journal      of Trace Elements in Medicine and Biology.

· 2023/01-2025/12, The role and mechanism of DNA methylation in the occurrence of ischemic stroke caused by copper exposure, Young Scientists Fund of National Natural Science Foundation of China, Grant No: 82203997 (300,000 RMB), PI.

· 2023/01-2024/12, The role and mechanism of DNA methylation in the association between polychlorinated biphenyl exposure and the risk of acute coronary syndrome, China Postdoctoral Science Foundation Funded Project, Grant No: 2022M721242 (80,000 RMB), PI.

· 2022/01-2026/12, A prospective Chinese cohort study of multi-omics and regulatory networks in atherosclerotic cardiovascular diseases, Major Program of the National Natural Science Foundation of China, Grant No:82192903 (4,750,000 RMB), core member (4/11).

· 2021/01-2025/12, Innovative research group for environmental chemicals and major chronic diseases, Science Fund for Creative Research Groups of the National Natural Science Foundation of China, Grant No: 82021005 (10,000,000 RMB), participating member.

· 2020/01-2024/12, Associations of multiple metals, metabolites, gene polymorphisms, DNA methylation, and their interactions with incident cardiovascular disease and the potential mechanism, Key Program of the National Natural Science Foundation of China, Grant No: 81930092 (2,970,000 RMB), participating member.

· 2016/7-2021/6, Prospective cohort study of common chronic noncommunicable diseases in Central China, National Key Research and Development Project of China, Grant No: 2016YFC0900800 (53,310,000 RMB), participating member.

Peer reviewed journal articles:

1. Yuan Y#, Long P#, Liu K, Xiao Y, He S, Li J, Mo T, Liu Y, Yu Y, Wang H, Zhou L, Liu X, Yang H, Li X, Min X, Zhang C, Zhang X, Pan A, He M, Hu FB, Navas-Acien A and Wu T. Multiple plasma metals, genetic risk and serum C-reactive protein: A metal-metal and gene-metal interaction study. Redox biology. 2020;29:101404. (co-first author)

2. Long P, Liu X, Li J, He S, Chen H, Yuan Y, Qiu G, Yu K, Liu K, Jiang J, Yang H, Xu C, Zhang X, He M, Guo H, Liang L, Hu FB, Wu T and Pan A. Circulating folate concentrations and risk of coronary artery disease: a prospective cohort study in Chinese adults and a Mendelian randomization analysis. Am J Clin Nutr. 2020;111:635-643.

3. Long P#, Wang Q#, Zhang Y, Zhu X, Yu K, Jiang H, Liu X, Zhou M, Yuan Y, Liu K, Jiang J, Zhang X, He M, Guo H, Chen W, Yuan J, Cheng L, Liang L and Wu T. Profile of copper-associated DNA methylation and its association with incident acute coronary syndrome. Clin Epigenetics. 2021;13:19. (co-first author)

4. Long P#, Wang H#, Zhang Z, Li W, Zhang Y, He S, Yu K, Jiang H, Liu X, Guo H, He M, Zhang X, Wu T and Yuan Y. Plasma metal concentrations and their interactions with genetic susceptibility on homocysteine levels. Ecotoxicol Environ Saf. 2022;241:113705. (co-first author)

5. Mo T#, Long P#, Wang Y, Peng R, Niu R, Wang Q, Jiang J, Shi L, Yang H, Xu C, Zhang X, He M, Guo H and Wu T. Genetic susceptibility, homocysteine levels, and risk of all-cause and cause-specific mortality: A prospective cohort study. Clin Chim Acta. 2023;538:1-8. (co-first author)

6. Mo T, Wang Y, Gao H, Li W, Zhou L, Yuan Y, Zhang X, He M, Guo H, Long P* and Wu T. Sleep Duration, Midday Napping, and Serum Homocysteine Levels: A Gene-Environment Interaction Study. Nutrients. 2023;15. (corresponding author)

7. Xu J, You Y, Yuan Y, Wang H, Wu T and Long P*. Associations of circulating multiple metals with the risk of incident hyperuricemia and the average annual change in uric acid levels. Ecotoxicol Environ Saf. 2023;262:115312. (corresponding author)

8. Yu L#, Long P#, Chen W and Wu T. Progress in research of deaths and disease burden of major chronic diseases caused by indoor and outdoor air pollution in China. Zhonghua Liu Xing Bing Xue Za Zhi. 2023;44:699-704. (co-first author)

9. Guo Y, Wang H, Xiao M, Guan X, Lei Y, Diao T, Long P, Zeng R, Lai X, Cai H, You Y, Wen Y, Li W, Wang X, Wang Y, Chen Q, Yang Y, Qiu Y, Chen J, Zeng H, Ni W, Zhao Y, Ouyang K, Wang J, Wang Q, Liu L, Song L, Wang Y, Guo H, Li X, Wu T and Yuan Y. Long-term outcomes of COVID-19 convalescents: An 18.5-month longitudinal study in Wuhan. Int J Infect Dis. 2023;127:85-92.

10. Jiang J, He S, Liu K, Yu K, Long P, Xiao Y, Liu Y, Yu Y, Wang H, Zhou L, Zhang X, He M, Guo H, Wu T and Yuan Y. Multiple plasma metals, genetic risk and serum complement C3, C4: A gene-metal interaction study. Chemosphere. 2022;291:132801.

11. Jiang J, Wang H, Liu K, He S, Li Z, Yuan Y, Yu K, Long P, Wang J, Diao T, Zhang X, He M, Guo H and Wu T. Association of Complement C3 With Incident Type 2 Diabetes and the Mediating Role of BMI: A 10-Year Follow-Up Study. J Clin Endocrinol Metab. 2023;108:736-744.

12. Jiang Q, Xiao Y, Long P, Li W, Yu Y, Liu Y, Liu K, Zhou L, Wang H, Yang H, Li X, He M, Wu T and Yuan Y. Associations of plasma metal concentrations with incident dyslipidemia: Prospective findings from the Dongfeng-Tongji cohort. Chemosphere. 2021;285:131497.

13. Li W, Xu X, Jiang Q, Long P, Xiao Y, You Y, Jia C, Wang W, Lei Y, Xu J, Wang Y, Zhang M, Liu C, Zeng Q, Ruan S, Wang X, Wang C, Yuan Y, Guo H and Wu T. Circulating metals, leukocyte microRNAs and microRNA networks: A profiling and functional analysis in Chinese adults. Environ Int. 2022;169:107511.

14. Liu K, Jiang J, Lin Y, Liu W, Zhu X, Zhang Y, Jiang H, Yu K, Liu X, Zhou M, Yuan Y, Long P, Wang Q, Zhang X, He M, Chen W, Guo H and Wu T. Exposure to polycyclic aromatic hydrocarbons, DNA methylation and heart rate variability among non-current smokers. Environ Pollut. 2021;288:117777.

15. Liu X, Shen M, Yan H, Long P, Jiang H, Zhang Y, Zhou L, Yu K, Qiu G, Yang H, Li X, Min X, He M, Zhang X, Choi H, Wang C and Wu T. Alternations in the gut microbiota and metabolome with newly diagnosed unstable angina. J Genet Genomics. 2022;49:240-248.

16. Mu X#, Yu K#, Long P, Niu R, Li W, Chen H, Gao H, Li X, Yuan Y, Yang H, Zhang X, He MA, Liu G, Guo H and Wu T. Leisure-time physical activity and risk of incident cardiovascular disease in Chinese retired adults. Scientific reports. 2021;11:24202.

17. Shen M, Xu X, Liu X, Wang Q, Li W, You X, Peng R, Yuan Y, Long P, Niu R, Yang H, Cheng X, Pan A, Tanguay RM, Zhang X, He M, Wang C, Liang L and Wu T. Prospective Study on Plasma MicroRNA-4286 and Incident Acute Coronary Syndrome. Journal of the American Heart Association. 2021;10:e018999.

18. Shi L, Yuan Y, Xiao Y, Long P, Li W, Yu Y, Liu Y, Liu K, Wang H, Zhou L, Yang H, Li X, He M and Wu T. Associations of plasma metal concentrations with the risks of all-cause and cardiovascular disease mortality in Chinese adults. Environ Int. 2021;157:106808.

19. Wang H, Gan M, Wu B, Zeng R, Wang Z, Xu J, Li J, Zhang Y, Cao J, Chen L, Di D, Peng S, Lei J, Zhao Y, Song X, Yuan T, Zhou T, Liu Q, Yi J, Wang X, Cai H, Lei Y, Wen Y, Li W, Chen Q, Wang Y, Long P, Yuan Y, Wang C, Pan A, Wang Q, Gong R, Fan X, Wu T and Liu L. Humoral and cellular immunity of two-dose inactivated COVID-19 vaccination in Chinese children: A prospective cohort study. J Med Virol. 2023;95:e28380.

20. Wang H, Yuan Y, Wu B, Xiao M, Wang Z, Diao T, Zeng R, Chen L, Lei Y, Long P, Guo Y, Lai X, Wen Y, Li W, Cai H, Song L, Ni W, Zhao Y, Ouyang K, Wang J, Wang Q, Liu L, Wang C, Pan A, Li X, Gong R and Wu T. Neutralization against SARS-CoV-2 Delta/Omicron variants and B cell response after inactivated vaccination among COVID-19 convalescents. Front Med. 2023:1-11.

21. Wang H, Yuan Y, Xiao M, Chen L, Zhao Y, Haiwei Z, Long P, Zhou Y, Xu X, Lei Y, Bihao W, Diao T, Cai H, Liu L, Shao Z, Wang J, Bai Y, Wang K, Peng M, Liu L, Han S, Mei F, Cai K, Lei Y, Pan A, Wang C, Gong R, Li X and Wu T. Dynamics of the SARS-CoV-2 antibody response up to 10 months after infection. Cell Mol Immunol. 2021;18:1832-1834.

22. Wu P, Ding L, Li X, Liu S, Cheng F, He Q, Xiao M, Wu P, Hou H, Jiang M, Long P, Wang H, Liu L, Qu M, Shi X, Jiang Q, Mo T, Ding W, Fu Y, Han S, Huo X, Zeng Y, Zhou Y, Zhang Q, Ke J, Xu X, Ni W, Shao Z, Wang J, Liu P, Li Z, Jin Y, Zheng F, Wang F, Liu L, Li W, Liu K, Peng R, Xu X, Lin Y, Gao H, Shi L, Geng Z, Mu X, Yan Y, Wang K, Wu D, Hao X, Cheng S, Qiu G, Guo H, Li K, Chen G, Sun Z, Lin X, Jin X, Wang F, Sun C and Wang C. Trans-ethnic genome-wide association study of severe COVID-19. Commun Biol. 2021;4:1034.

23. Xu X, Ma J, Li W, You Y, Jiang Q, Long P, Liu K, Mo T, Jiang J, Wang W, Lei Y, Diao T, Ruan S, Wang X, Guo H, Chen W and Wu T. Polycyclic aromatic hydrocarbons exposure and plasma lncRNA signature: A profile and functional analysis. Sci Total Environ. 2023;877:162932.

24. Wang X, Yin Y, Wang H, Long P, Chen W, Yuan Y and Wu T. Progress in research of relationship between metal or metalloid and persistent organic pollutants exposures and cardiovascular disease. Zhonghua Liu Xing Bing Xue Za Zhi. 2023;44:705-712.

Journal articles in preparation:

Pinpin Long, et al. Genome-wide DNA methylation profiling in blood reveals epigenetic signature of incident acute coronary syndrome. Circulation. (Under review)

Participating monograph:

Environment and Precision Prevention, Shanghai Jiao Tong University Press, Editor-in-Chief Tangchun Wu, participated in the preparation of Chapter 7, "Proteomics in Precision Health Research", published in the first edition of December 2018.

Patent for invention:

DNA methylation patterns associated with incident acute coronary syndrome and their applications, Patent publication number: CN116622834A, invention application, my position: 3/9.

Postgraduate stage

· National scholarship for doctoral students, HUST, China (November 2020)

· Merit graduate student, HUST, China (November 2020)

· The Third Prize Scholarship, HUST, China (November 2020)

· Party branch secretary model, HUST, China (September 2020)

· The Third Prize Scholarship, HUST, China (November 2019)

· Merit graduate student, HUST, China (November 2018)

· The Third Prize Scholarship, HUST, China (November 2017)

· Merit graduate student, HUST, China (November 2017)

Undergraduate period

· Outstanding Graduates, Sichuan University, China (November 2015)

· National Encouragement Scholarship, Sichuan University, China (November 2015)

· National scholarship for undergraduate, Sichuan University, China (November 2014)

· College students medical health inspection and quarantine knowledge and skills competition, Sichuan University, China (November 2014)

· Merit student, Sichuan University, China (November 2013)

· The Second Prize Scholarship, Sichuan University, China (November 2013)